rabbit polyclonal antibody  (Novus Biologicals)

Journal: Biomedicines

Article Title: Multi-Transcriptomic Analysis Reveals GSC-Driven MES-Like Differentiation via EMT in GBM Cell–Cell Communication

doi: 10.3390/biomedicines13061304

Figure Lengend Snippet: GSCs exhibit pronounced overexpression of EMT-associated gene signature in GBM. ( A ) Distribution of all GSCs ( n = 65,655), including 28 early-passage GSC cultures derived from 24 patients and all tumor cells ( n = 14,207) from seven patients with GBM. ( B ) Quantification of hallmark of EMT in scRNA-seq data. Violin plots represent the overall distribution of data points. Box plots show median, upper, and lower quartiles; whiskers depict 1.5 times the interquartile range. ( A , B ) Data sourced from the Broad Institute Single-Cell Portal ( https://singlecell.broadinstitute.org/single_cell/study/SCP503 , accessed on 17 March 2025) and CReSCENT60 ( https://crescent.cloud ; accessed on 16 March 2025, study ID CRES-P23). ( C ) Immunoblots showing BIRC5, VIM, CTNNB1, and SNAI2 expression in the indicated cells. GFAP is a differentiation marker, and SOX2 is a stem cell marker. Immunoblots are representative of three independent experiments with similar results. ( D – G ) H3K27ac ChIP-seq tracks at BIRC5, VIM, CTNNB1, and SNAI2 gene loci. ( D – G ) Data sourced from the GEO dataset ( GSE129438 ).

Article Snippet: SURVIVIN (BIRC5) polyclonal antibody , Proteintech , 10508-1-AP.

Techniques: Over Expression, Derivative Assay, Western Blot, Expressing, Marker, ChIP-sequencing

Journal: The Indonesian Biomedical Journal

Article Title: Elephantopus scaber Linn. Leaf Extract Sensitizes Doxorubicin in Inducing Apoptosis in HSC-3 Tongue Cancer Cells through Inhibiting Survivin Activity at Thr34

doi: 10.18585/inabj.v16i4.3096

Figure Lengend Snippet: Figure 4. ESLE decreased the amount of p-Surv (Thr34) in HSC-3 cells in concentration-dependent manner. HSC-3 cells were treated with/without 25 nM YM155 or ESLE in various concentrations. The amount of p-Surv (Thr34) was measured using Western Blot, as outlined in Methods. *Statistical significance (p<0.05) was determined using Tukey’s post-hoc test when compared to the sham group.

Article Snippet: After blocking with 5% skim milk in Tris-buffered saline (TBS, 150 nM NaCl and 50 nM Tris-HCl, pH 7.4), the sheets were incubated with rabbit polyclonal anti-β-Actin (Cat. No. 4967; Cell Signaling, Danvers, MA, USA) and rabbit polyclonal anti-phospho-survivin (Thr34) (Cat. No. 8888; Cell Signaling) antibody.

Techniques: Concentration Assay, Western Blot